Another method known as 3D cell culture has shown improvements in studies targeted toward morphology, cell number monitoring, proliferation, response to stimuli, differentiation, drug metabolism, and protein synthesis ( Antoni et al., 2015). Two dimensional (2D) cell culture has been the method used to culture cells since the early 1900s ( Ferreira et al., 2018), which plays a vital role in research but has many limitations due to 2D models inaccurately representing tissue cells in vitro ( Costa et al., 2016). The present review compares 2D cell culture to 3D cell culture, provides the details surrounding the different 3D culture techniques, as well as focuses on the present and future applications of 3D cell culture. 3D cell culture has the potential to provide alternative ways to study organ behavior via the use of organoids and is expected to eventually bridge the gap between 2D cell culture and animal models. Likewise, there are also scaffold free techniques used such as hanging drop microplates, magnetic levitation, and spheroid microplates with ultra-low attachment coating. Scaffold based techniques such as hydrogel-based support, polymeric hard material-based support, hydrophilic glass fiber, and organoids are employed, and each provide their own advantages and applications. When performing 3D cell culture experiments, the cell environment can be manipulated to mimic that of a cell in vivo and provide more accurate data about cell-to-cell interactions, tumor characteristics, drug discovery, metabolic profiling, stem cell research, and other types of diseases. Most cells are currently cultured using two-dimensional (2D) methods but new and improved methods that implement three-dimensional (3D) cell culturing techniques suggest compelling evidence that much more advanced experiments can be performed yielding valuable insights. 5Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta University, Augusta, GA, United StatesĬell culture is an important and necessary process in drug discovery, cancer research, as well as stem cell study.4Department of Medical Laboratory, Imaging and Radiologic Sciences, College of Allied Health, Augusta University, Augusta, GA, United States.3Georgia Cancer Center, Medical College of Georgia, Augusta University, Augusta, GA, United States.2Department of Biology, College of Science and Mathematics, Augusta University, Augusta, GA, United States.1Department of Oral Biology and Diagnostic Sciences, Dental College of Georgia, Augusta University, Augusta, GA, United States.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |